TABLE 3.

Transcription in various nuclear preparations

	UTP incorporation (pmole/106 nuclei/10 min)
	CHO	HeLa (Iborra et al. 2001)	HeLa (purified)
Untreated	32.2	12.6	11.0
Pretreatment with DNase	3.3	2.8	0.6
Product treated with RNase	1.6	1.0	0.4

Incorporation of [α′-³²P] UTP into RNA using isolated nuclei preparations was carried out as described in Materials and Methods. DNase treatment was at 28°C for 30 min prior to addition of NTPs using 5 units of RNase-free DNase. RNase treatment was at 28°C for 30 min after completion of synthesis using 100 μg/ml RNase A. (CHO) Chinese hamster ovary.