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. 2003 Jan;9(1):112–123. doi: 10.1261/rna.2780503

FIGURE 4.

FIGURE 4.

Inhibition of luciferase expression by miR-30 and miR-21. (A) Schematic of the luciferase indicator plasmids bearing four miR-30 or miR-21 target sequences (black boxes). (B) miR-30 inhibits the expression of the luciferase indicator bearing 4xmiR-30 target sites. A dual luciferase assay was performed, and the ratio of firefly luciferase to the internal control Renilla luciferase, from cells cotransfected with the parental pBC12/CMV vector, was set at 1 (shown by −). (C) miR-21 inhibits the expression of the luciferase indicator encoding 4xmiR-21 target sites. Descriptions are the same as in B. (D,E) Northern analyses detecting the luc indicator mRNA, or the β-gal internal control mRNA, in 293T cells cotransfected with the pBC12/CMV control (−) or the indicated miRNA expression plasmid.