TABLE 2.
CAT activity of CRE-containing replicons
| Replicon cDNA template | Activity |
| pT7/Rep3 | +++ |
| pT7/Rep3/SL3 | − |
| pT7/Rep3/SL3/#1 | + |
| pT7/Rep3/SL3/#3 | + |
| pT7/Rep3c | − |
| pT7/Rep3cWT | + |
| pT7/Rep3cSL3 | + |
| pT7/Rep3/SL3c | − |
| pT7/Rep3/SL3cWT | + |
| pT7/Rep3/SL3cSL3 | − |
| pT7/Rep3/SL3cΔStem1 | − |
| pT7/Rep3/SL3cΔStem1+2 | − |
| pT7/Rep3/SL3cInv1 | + |
| pT7/Rep3/SL3cInv3 | + |
| pT7/Rep3/SL3cClal | + |
| pT7/Rep3/SL3cSynth2 | +++ |
| pT7/Rep3/SL3cSynth3 | +++ |
| pT7/Rep3/SL3cSynth4 | +++ |
| pT7/Rep3/SL3cSynth5 | +/− |
| pT7/Rep3/SL3cSynth6 | − |
Notes: CAT activity indicated in the range +++ (100%; native activity of Rep3 replicon) to − (no detectable activity). + indicates ∼30% wild-type activity relative to the pT7/Rep3 replicon. See also Figure 3B ▶ and Figure 3D ▶. We have previously quantified both the RNA levels and the resulting CAT activity of replicating RNA generated from pT7/Rep3/SL3/#1 and pT7/Rep3/SL3/#1 (Goodfellow et al. 2000).