TABLE 1.

RRE-binding peptides identified from an oligoarginine-based library using the LacZ reporter

				X-gala		ONPGb
Clone #	Peptide sequence			RRE	(BTAR)	RRE	(BTAR)
	Dark Blue (4+)
1	MA	PRRDRRRRRQ	RRRRRAAAA	4	(0)	29.3	(4.2)
2	MA	DRADRRRRRQ	RRRRRAAAV	4	(0)	28.3	(3.7)
3	MA	PRNQRRRRRQ	RRRRRAAAA	4	(0)	28.2	(3.6)
4	MA	RRRDRRRERQ	RRRRRAAAA	3	(0)	21.0	(3.4)
	Blue (3+)
5	MA	RTRRERRHRQ	RRRRRAAAT	3	(0)	18.4	(3.3)
6	MA	GRADRRHRRQ	RRRRRAAAA	3	(0)
7	MA	PRTRRRRRRQ	RRRRRAAAA	3	(0)
8	MA	TRRRRRRNRQ	RRRRRAATS	3	(0)
9	MA	GTTQRRRTRQ	RRRRRAAAA	3	(0)	12.2	(2.8)
10	MA	TRRDRRRARQ	RRRRRAAAA	3	(0)
Rev	MA	TRQARRNRRRRWRRAAAA		3	(0)	26.2	(5.0)
RSG-1.2	MA	RRRRRRGSRPSGAERRRRRAAAA		4	(0)	36.6	(5.3)
R6QR7c		AAAARRRRRRQRRRRRRRAAAA

^aX-gal colony assays were performed as described in Peled-Zehavi et al. (2000). Numbers represent plusses used to score blue color intensity. Values in parentheses indicate activities with the nonspecific BIV TAR reporter.

^bβ-Galactosidase solution assays were performed as described in Peled-Zehavi et al. (2000). Values in parentheses indicate activities with the nonspecific BIV TAR reporter.

^cThe sequence of the R6QR7 peptide identified in a mammalian screening assay (Tan and Frankel 1998) is shown for comparison but experiments with this peptide were not performed in the antitermination assay.