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The acceptor-stem sequences of active mutants selected from libraries. The shaded bases in the cloverleaf of yeast tRNAAsp were randomized. E. coli-specific bases are in solid black font, yeast-specific bases are in outline, new bases are underlined in dotted outline, and common bases are gray. All yeast tRNAs were designed to contain the E. coli C32 and A37 nucleotides (see text). Modified bases in the cloverleaf structure are not indicated.
