FIGURE 9.

Northern blot analysis of total RNA incubated with RNase P and RNase E. RNAs (10 μg) from A45 were incubated at 37°C for 15 min with RNase P (0.05 μM M1 RNA and 0.5 μM C5 protein) or with 2.5 μg/mL of RNase E. The reaction was terminated by addition of phenol/9 M urea and applied to a 6% polyacrymide gel. Northern blot analysis was then performed using the oligonucleotides probing for hisB, tnaA and rbsB. (Lane l) control reaction without enzymes; (lane 2) total RNAs with RNase P; (lane 3) total RNAs with RNase E; (lane 4) total RNAs with RNase P and RNase E.