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. 2003 May;9(5):555–565. doi: 10.1261/rna.2190803

FIGURE 3.

FIGURE 3.

Effects of GNRAR15 and AAAAR15 and its derivatives on RNA synthesis. (A) Sequences and predicted structures of GNRAR15, AAAAR15, and its derivatives. The names and predicted ΔG values for each RNA are shown below the sequences. The arrows show the initiation cytidylates. (B) RNA synthesis directed by GNRAR15, AAAAR15, and its derivatives. The size of the replicase products, in nucleotides, is shown to the left of the autoradiogram. Asterisks denote the expected positions of the 28-, 28-, 24-, 22-, and 20-nt products from GNRAR15, AAAAR15, AAAAR15m1, AAAAR15m2, and AAAAR15m3, respectively. The percentages of synthesis are normalized to number of radiolabeled cytidylates incorporated relative to the control; they are from two replicates. (C) Results from thermodenaturation analysis. A first derivative of the absorbance at 260 nm is plotted as a function of temperature. The Tm of GNRAR15 is 78°C, AAAAR15 is 57°C, AAAAR15m1 is 50°C, AAAAR15m2 is 40°C, and AAAA15m3 is 16°C. (D) RNAs visualized after electrophoresis of 40 pmole of RNA in a 7 M urea PAGE (upper image) and 120 pmole of RNA in a nondenaturing PAGE (lower image).