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. 2003 May;9(5):555–565. doi: 10.1261/rna.2190803

FIGURE 6.

FIGURE 6.

Subgenomic RNA synthesis. (A) Sequences and structures of subgenomic RNAs. −20/13 is a 33-nt RNA containing a 20-nt core subgenomic promoter and a 13-nt template. Arrows show the mutations from the −13 C to an A, which should abolish RNA synthesis (Siegel et al. 1998). The GNRA tetraloop is shown as stems attached to the 3′ or 5′ termini of the subgenomic proscript. An extra uridylate (lower case “u”) is added at the 3′ terminus to provide a one-nucleotide spacer from the core promoter to the tetraloop stem. The prefix or suffix of GNRA indicates whether the tetraloop stem is placed at the 3′ or 5′ of the subgenomic proscript. Bent arrows show the initiation sites for RNA synthesis. (B) Autoradiogram of the RNAs synthesized by the BMV replicase from the templates shown in (A). Sizes of synthesis products are shown beside the figures. Quantified results (below the lanes) are normalized to number of radiolabled cytidylates incorporated relative to the control and are from three independent replicates.