FIGURE 8.

GNRAR15 does not cause premature termination of RNA synthesis by the BMV replicase. Products for RNA synthesis were separated on a 25% polyacrylamide–7 M urea gel. The templates for the reaction are shown above the autoradiogram. Sequences for R15 and GNRAR15 were presented in Figures 2A and 3A ▶ ▶. The sequence of the initiation-incompetent RNA, R15init-, is shown above the autoradiogram. “M” denotes products made by the T7 RNA polymerase from a DNA template that should generate identical products to the ones made by the BMV replicase from R15. The sizes of the products from the T7 RNA polymerase are indicated to the right of the autoradiogram. The sizes of the products that could be made by the BMV replicase are indicated to the left of the autoradiogram. The asterisk identifies the faint bands produced by the BMV replicase using GNRAR15 as a template.