FIGURE 4.

Editing activity per molecule of the N-terminal deletion mutant Δ5–133 and ADAR1-S in vitro. (A) ADAR1-S and Δ5–133 purified from HEK293 cells. Proteins were electrophoresed on a 10% SDS–polyacrylamide gel and stained with Coomassie blue (left) or immunoblotted with anti-HA monoclonal antibody (right). Lane 1 is Δ5–133, and lane 2 is ADAR1-S; the dots mark their positions. (B) In vitro editing assay. Decreasing concentrations of Δ5–133 (lanes 2–6) and ADAR1-S (lanes 7–11) were added to HDV antigenomic RNA. Sty I-digested reverse transcription–PCR products of the in vitro editing reaction were resolved on an agarose gel. *U is the unedited product, and *E1 and *E2 are the two products of editing. MW is the molecular-weight marker (lane 1). The percentage of editing was calculated by E1+E2/U+E1+E2. (C) A graphical representation of the average of two in vitro editing experiments.