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. 2003 May;9(5):607–617. doi: 10.1261/rna.5250403

FIGURE 2.

FIGURE 2.

Introns enhance both luciferase expression and mRNA accumulation in transiently transfected HeLa cells. (A) Cell lysate luciferase activity measured 42 h post-transfection. Activities shown are an average of three independent experiments; error bars represent standard deviation. (B) Time course of luciferase expression in living cells. Transfected HeLa cells were monitored for luciferase activity from 4 to 46 h post-transfection. (C) Analysis of total TPI/Renilla mRNA levels 42 h post-transfection. RPA reactions contained 250 ng or 1 μg total RNA using probe A and probe C (schematized in Fig. 1A ▶). Bands corresponding to pre-mRNA and spliced mRNA are as indicated. Doublet observed with probe A is due to partial homology in the noncomplimentary tail of the probe. Zeocin mRNA, which is transcribed from the same plasmid as TPI/Renilla mRNA was used as a control. (UT) Untransfected. (D) Quantitation of mRNA levels. TPI/Renilla mRNA levels were normalized to zeocin mRNA and plotted relative to the no-intron control. Relative mRNA levels reflect an average of three independent experiments; error bars represent standard deviation.