FIGURE 4.

Translation of newly made mRNA is independent of the eIF4G-CBC interaction. The yeast strains 4G1 and 4G1–548 were transformed with a plasmid expressing the luciferase reporter mRNA under the control of a galactose-inducible promoter. The resulting strains YBJ 220 and YBJ 221, respectively, were used to monitor the accumulation of luciferase activity over time after galactose induction as described in Materials and Methods. The luciferase activity was normalized against the number of cells per sample. The graph represents the average of three independent experiments.