TABLE 1.

Yeast strains used in this study

Systematic name	Name used in this study	Disrupted interaction	Plasmids
YAS 2632 (α)	4G1	none	TRP1-CEN-pTIF4631
YAS 2757 (α)	4G1-548	CBC	TRP1-CEN-ptif4631-548
YAS 2074 (β)	4G1-459	eIF4E	TRP1-CEN-ptif4631-459
YAS 2758 (α)	4G1-459,548	eIF4E, CBC	TRP1-CEN-ptif4631-459,548
YBJ 220 (δ)		none	TRP1-CEN-pTIF4631 URA-CEN-pGal-Luc
YBJ 221 (δ)		CBC	TRP1-CEN-ptif4631-548 URA-CEN-pGal-Luc
Name	Genotype
Δcbp80	MATa ade2 ade3 his3 leu2-3,112 trp1 ura3 ycbp80/gcr3::TRP1
Δcbp20	MATa ade2 ade3 his3 leu2-3,112 trp1 ura3 ycbp20/mud13::HIS3

α: These strains have the following genotype: MATa ade2-1 his3-11,15 leu2-3,111 trp1-1 ura3-1 pep4::HIS3 tif4631::leu2 tif4632::ura3.

β: This strain is described by Tarun and Sachs (1997); it has the following genotype: MATa ade2-1 his3-11,15 leu2-3,111 trp1-1 ura3-1 pep4::HIS3 tif4631::LEU2.

δ: YBJ 220 and YBJ 221 derived respectively from the strains YAS 2632 and YAS 2757.