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. 2003 Jun;9(6):698–710. doi: 10.1261/rna.2120703

FIGURE 3.

FIGURE 3.

Diagrammatic alignment of compiled genomic sequence organization and sas-10 transcription unit characteristics. Orientation diagrams at top and bottom show relative locations of genes sas-10 and 4f-rnp, separated by 95-bp intergenic spacer (S), with their corresponding leader (L) and conventional trailer (T) tracts. Within poly(A) cDNA clones 4R1#6 (Hunt-Smith 1999) and GH08670 (Drosophila EST database), which both initiate transcription at promoter P1, locations of 5′-termini relative to start codon AUG, putative poly(A) signals (AAUAAA; AAUAUA) relative to 3′-termini, and transcript lengths relative to the start codon (+1) are indicated. Sas-10 transcripts determined by RT-PCR as arising from promoter P2 are labeled with the PCR primers utilized. Their numbering scheme is as for the cDNA clones, and the extent of their final length in vivo is unknown. cDNA clone GH08670 and the shorter RT-PCR transcript depicted overlap with the 4f-rnp trailer to varying extents, while the longer RT-PCR transcript depicted also overlaps the final 251 nt of the 4f-rnp coding region.