FIGURE 7.

Rescue of the A248U miscleavage phenotype by the RNase P holoenzyme. (A) Resolution of ligated substrate cleavage products. Combinations of enzyme and substrate are as indicated. Correct (AAAAU) and incorrect (AAAA) cleavage products are indicated. Reactions were run under holoenzyme conditions as described in the Materials and Methods. (B) Bar graph showing cleavage site preference for native and A248 mutant enzymes in the holoenzyme reaction. Correct cleavage of dA(-1) substrate is shown in white; dU(-1) bars are cross-hatched. Error bars represent the standard deviation of three or more independent determinations.