TABLE 2.
Site | Organism | Mutants used | Heptameric sequencea | tRNA in P-site | Modification tested in P-site tRNA | tRNA in A-site | Modification tested in A-site tRNA |
HIV | S. typh. | miaA | U-UUU-UUA | tRNAPheGAA | ms2io6- of ms2io6A37 | tRNALeucmnm5UmAA | ms2io6- of ms2io6A37 |
HIV | S. typh. | miaB | U-UUU-UUA | tRNAPheGAA | ms2- of ms2io6A37 | tRNALeucmnm5UmAA | ms2- of ms2io6A37 |
HIV | S. typh. | miaE | U-UUU-UUA | tRNAPheGAA | o6- of ms2io6A37 | tRNALeucmnm5UmAA | o6- of ms2io6A37 |
HIV | S. typh. | truA | U-UUU-UUA | tRNAPheGAA | Ψ39 | tRNALeucmnm5UmAA | Ψ39 |
HIV | E. coli | truB | U-UUU-UUA | tRNAPheGAA | Ψ55 | tRNALeucmnm5UmAA | Ψ55 |
argI | S. typh. | miaA, miaB | G-UUU-UAU | tRNAPheGmAA | ms2io6- of ms2io6A37 | tRNATyrQUA | ms2io6- of ms2io6A37 |
argI | S. typh. | tgt | G-UUU-UAU | tRNAPheGmAA | None | tRNATyrQUA | Q34 |
MMTV | E. coli | mnmA | A-AAA-AAC | tRNALysmnmn5s2UUU | s2- of mnm5s2U34 | tRNAAsnQUU | None |
MMTV | E. coli | mnmE | A-AAA-AAC | tRNALysmnmn5s2UUU | mnm5- of mnm5s2U34 | tRNAAsnQUU | None |
MMTV | S. typh. | tgt | A-AAA-AAC | tRNALysmnmn5s2UUU | None | tRNAAsnQUU | Q34 |
MMTV | E. coli | truA | A-AAA-AAC | tRNALysmnmn5s2UUU | Ψ39 | tRNAAsnQUU | Ψ39 |
MMTV | E. coli | truB | A-AAA-AAC | tRNALysmnmn5s2UUU | Ψ55 | tRNAAsnQUU | Ψ55 |
HIV | S. cerev. | trm5 | U-UUU-UUA | tRNAPheGmAA | Y-base | tRNALeuU?AA | m1G37 |
L-A | S. cerev. | trm5 | G-GGU-UUA | tRNAGlyU?CC | None | tRNALeuU?AA | m1G37 |
aThe first triplet denotes the codon in the P-site and the next triplet the codon in the A-site, if the frameshifting occurred as postulated by the “simultaneous slippage model.”