FIGURE 2.

Depletion of CeNXT1/p15 causes accumulation of poly(A)⁺ mRNA in the nuclei. Injected animals and control N2 worms were fixed and subjected to RNA in situ hybridization as described. In each panel the intestine is the predominant tissue characterized by the presence of large nuclei. Accumulation of poly(A)-containing RNAs was observed in intestinal nuclei of RNAi-treated animals (indicated by arrows in b and d) and corresponding DAPI-stained nuclei as shown in a and c. Poly(A)⁺ RNA distribution in intestines of wild-type, untreated worms are shown in f and h (nuclei are indicated by arrowheads), and corresponding DAPI- stained nuclei are shown in e and g. Bar, 25 μm.