FIGURE 4.

RNAi depletion of C. elegans Ref/Aly genes mRNAs is efficient and specific. The level of the residual transcripts following dsRNA injections was examined by RT–PCR with specific primers, as described previously (Longman et al. 2000) CeRef 1, 2, and 3 mRNAs are specifically depleted in RNAi treated animals (lanes 6,7,8, respectively) compared with wild-type animals (lanes 2,3,4, respectively), whereas the level of a control mRNA corresponding to CeNXF1/Tap1 gene is unaffected (lanes 1,5). The figure shows a negative of an ethidium bromide-stained agarose gel. (M) 100-bp ladder DNA size marker.