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. 2003 Jul;9(7):892–904. doi: 10.1261/rna.5440303

FIGURE 2.

FIGURE 2.

RNA X formation is a reversible reaction. (A) RNA X formation can be reversed. Identity and concentration of the RNA or DNA oligonucleotide added to each reaction is shown above each lane. RNA products were analyzed by denaturing PAGE and detected by a PhosphorImager. The position of RNA X is indicated. No oligonucleotide was added to the reaction marked Ctrl. (B) Purified RNA X is resistant to reversal. The positions of RNA X and Br are shown. (Ctrl) No DNA or RNA oligonucleotide added. Identity and concentration of the DNA oligonucleotide added is shown above the left lane. (C) αBr-mediated RNA X reversal requires U2. The identity of the DNA oligonucloetides added to each lane is indicated. No oligonucleotide was added to the sample in the lane marked Ctrl. Position of RNA X is shown to the right. The band marked by an asterisk is a degradation product of RNA X.