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. 2003 Aug;9(8):923–930. doi: 10.1261/rna.5510503

FIGURE 2.

FIGURE 2.

Sequences of C. reinhardtii Sec tRNA and other RNAs. (A) Cloverleaf model of Sec tRNA[Ser]Sec shown in a 9/4 representation (see text). The base at position 58 was determined to be T, but this position is shown as an A for reasons discussed in the text. The GenBank accession number for the nucleotide sequence of Sec tRNA[Ser]Sec is AY268554. (B) Sequences of other tRNAs. The upper six RNAs—Arg, Asp, Cys, Pro, Thr-1, and Thr?—are new sequences; the lower three—Asn, Thr-2, and Trp—which are designated with *, were reported previously (Yu et al. 1992; Maul et al. 2002). The sequence designated Thr? is an unknown RNA, but possibly a Thr tRNA (see text), and the sequences of the three previously reported tRNAs match those shown in the figure with the exception of the bracketed base at position 16 in Thr-2 tRNA (see text). Some of the sequences, indicated by #, are partial. Each tRNA sequence is aligned at the anticodon, which is in italics and underlined. The GenBank accession numbers for the nucleotide sequences of the new RNAs are AY268556 (Arg), AY268558 (Asp), AY268559 (Cys), AY268555 (Pro), AY268557 (Thr-1), and AY268560 (Thr?). (C) Number of times each tRNA clone was sequenced among 47 independently selected bacterial colonies from each fraction (see Fig. 1 ▶).