FIG. 7.

Cells treated with MG132 display a higher percentage of IE⁺ BrdU⁺ cells at 8 to 12 hpi. S-phase infections were set up on coverslips. Cells were pulsed with BrdU at 23 h postplating for 30 min, followed by incubation without label for 30 min. At 24 h postplating, cells were mock or virus infected in the presence or absence of 2.5 μM MG132 (or an equivalent amount of DMSO carrier). At 8 to 12 h later, cells were harvested, fixed, and processed for immunofluorescence using Abs for both BrdU and IE antigens as described in Materials and Methods. These results are representative of the data shown in Table 1.