FIGURE 3.
RRE IIB footprinting studies of the Rev peptides binding sites on the RNA construct. (A) Lead acetate footprinting. (B) RNase T1 footprinting. (C) RNase A footprinting. (0) Untreated RRE IIB, 32P-labeled at 5′; (Ctrl) control experiment in the absence of binders; (L-Rev, D-Rev, L-RevErse, D-RevErse) RNA footprinting in the presence of peptide binders; (NeoB) RNA footprinting in the presence of neomycin B. Concentrations of the peptides, 2.5 and 12.5 μM. Concentration of neomycin B 25 and 125 μM.