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. Author manuscript; available in PMC: 2026 Oct 27.
Published in final edited form as: Mol Biol Cell. 2026 Oct 26;37(11):ar105. doi: 10.1091/mbc.E25-08-0391

Figure 1.

Figure 1.

Cyclin E overexpression causes an increase and dependency on USP37. (A, B, C) USP37 protein expression in immortal and transformed cells of the colon, breast and lung. (D) Pearson correlation analysis of expression of USP37 versus CCNE1 and CCNE2 in the DepMap portal data. (E) USP37 gene effect score (DepMap Public 24Q4 +Score, Chronos) from the DepMap CRISPR dataset for cells with (3 or more copies – relative to ploidy) or without amplification of CCNE1 or CCNE2. Statistical significance was determined using an unpaired one-tailed t test (*, p<0.5). (F, G) Expression of USP37 and Cyclin E in HCT116 cells with the indicated genotypes. (F) and quantitation of USP37 protein (G), Statistical significance was determined using an unpaired two-tailed t test (*, p<0.5), n=3. (H, I) USP37 protein expression was analyzed in a cycloheximide (CHX) chase assay (H) and quantitation of USP37 protein (I), n=3. (J, K) Representative images (J) and quantification (K) of HCT116 and HCT116FBW7−/− colony formation after transfection with the indicated siRNAs. Bar graphs represent the mean and sd, n=3. Statistical significance was determined using two-way ANOVA and Holm-Sidak post-test (*, p<0.5).