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. Author manuscript; available in PMC: 2026 Oct 27.
Published in final edited form as: Mol Biol Cell. 2026 Oct 26;37(11):ar105. doi: 10.1091/mbc.E25-08-0391

Figure 5.

Figure 5.

USP37 stabilizes and deubiquitinates TIPIN. (A, B) Immunoblot analysis of 293T cells transfected with Flag-TIPIN and either empty vector, Myc-USP37C350A, or Myc-USP37 and analyzed by immunoblot after treatment with 100 ug/ml cycloheximide (CHX) (A) and quantification of TIPIN expression (B). Graph represents the mean and sd, n=3. Statistical significance was determined a two-way ANOVA with Holm-Sidak post-test compared to vector (*p<0.05, **p<0.01). (C) Immunoblot analysis of 293T cells transfected with Flag-TIPIN and an increasing amount of Myc-USP37. One sample was treated with 10 μM MG132 for 4hrs. (D) 293T cells were transfected with His-Ubiquitin (Ub), Myc-TIPIN, and USP37-Flag or USP37C350A-Flag, as indicated, and treated with 10 μM MG132 for 4hrs. Ubiquitin conjugated proteins were enriched by Ni+2 affinity purification followed by immunoblot for Myc or His epitopes. TPS = total protein stain, WCL = whole cell lysate.