Figure 6.

Expression of TIPIN attenuates S-phase progression and replication defects in USP37-deficient cells. (A) Representative images of cells transfected with the indicated siRNAs and either empty vector or FLAG-S-TIPIN 7 hours after a 10 μM EdU pulse. (B) Quantification of the Cyclin A-negative fraction of Edu-positive cells (CycA-,EdU+) cells, treated as in A. The bar graph represents the mean and sd, n=3. Statistical significance was determined a two-way ANOVA with Holm-Sidak post-test compared to vector (*p<0.05, **p<0.01). (C) Quantification of DNA fiber length in Hela cells after transfection with the indicated siRNAs and doxycycline-induced expression of the indicated transgenes. Red lines signify mean fiber length. Statistical significance was determined with a one-way ANOVA and Holm-Sidak post-test. (**p<0.01, ****p<0.0001).