FIG. 6.

Relative IFN sensitivity of early- and later-passage SeV-AGP65/GFP. BF and BHK-LR cell cultures were treated (or not) with 1,000 U of IFN-α2/α1 (49) for 16 h and then infected with 20 infectious units/cell of either VSV/GFP, SeV-wt/GFP, or SeV-AGP65/GFP at passage level 2 (P2) or 4 (P4). The cultures were harvested at 8 p.i. (VSV) or 48 h p.i. (SeV) and analyzed directly for green fluorescence by fluorescence-activated cell sorting. The M1 bar that denotes GFP-positive cells was fixed relative to uninfected cells, and their percentage of the population (−/+ IFN) is shown in each panel.