FIGURE 6.

Translational efficiency of ARCA-capped mRNAs. Luciferase mRNAs were synthesized in vitro using T7 RNA polymerase and SmaI-digested pluc-A+ in the presence of all four NTPs and Gp₃G (▪), m⁷Gp₃G (•), m⁷Gp₄G (first synthesis; ♦), m⁷Gp₄G (second synthesis; ▾), m₂^7,3′-OGp₄G (compound 14; ▴), or m₂^7,2′-OGp₄G (compound 15; ○), as described in Materials and Methods. The RNAs were translated for 60 min in a rabbit reticulocyte lysate system, and luciferase activity was measured in triplicate in 1-μL aliquots by luminometry; (RLU) relative light units.