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. 2003 Sep;9(9):1157–1167. doi: 10.1261/rna.5720303

FIGURE 5.

FIGURE 5.

Impact of inhibiting translation on steady-state levels of human β-globin mRNA in transfected MEL cells. The MEL cell transfectants described in the legend to Figure 4 were induced with DMSO for 48 h. At time 0, the medium the cells either received fresh medium (A) or medium containing cycloheximide (B). Cytoplasmic RNA was isolated at the indicated time points and analyzed by Northern blot for human β-globin mRNA (top, each set) and GAPDH (bottom, each set).