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. 2002 Jun;76(11):5326–5338. doi: 10.1128/JVI.76.11.5326-5338.2002

FIG. 4.

FIG. 4.

(A) Only a homopolymeric uridine tract in the 3′ NTR supports RNA replication. Luciferase replicons carrying 26-nucleotide homopolymeric guanosine, adenosine, uridine, or cytidine tracts were transfected into Huh-7 cells, and RNA replication was determined after 24, 48, and 72 h. Bars, mean values of quadruplicate determinations and the error ranges. Values are normalized for transfection efficiency as determined from the luciferase activities measured 4 h after transfection (set at 100%). Wt, wild-type replicon; GND, replication-defective replicon that was used as a negative control. (B) Full-length 3′X is required for RNA replication. Luciferase replicons that lack SL1, SL2, or SL3, or the complete 3′X were transfected in parallel with the parental replicon (Wt) or the replication-deficient mutant (GND) into Huh-7 cells, and transient replication was determined as described above.

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