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. 2003 Oct;9(10):1274–1281. doi: 10.1261/rna.5980303

FIGURE 3.

FIGURE 3.

Characterization of developmentally regulated miR-9 and miR-131 transcripts. (A) Northern blots of total RNA isolated from cerebral cortex (co), cerebellum (ce), kidney (ki), heart (ht), liver (li), lungs (lg), ovary (ov), thymus (thy), and testis (te), probed with miR-9 and mir-131. (B) Predicted secondary structures for putative precursors of multicopy human and rodent miR-9 and miR-131 cotranscribed pair. RNA secondary structure prediction was performed using mfold software. MiR-9 and miR-131 sequences are shown in bold. (C) Northern blots of total RNA isolated from pre- and postnatal developing brain with probes specific for the loop region of the predicted mir-9/miR-131 precursors. Oligonucleotides used as probes were 5′-TTATGAAGACTCCA CACCAC-3′ for chromosome 1 precursor, 5′-TTTATGAAGACCAATACAC-3′ for chromosome 5 precursor, and 5′-TTATGACGGCTCTGTGGCAC-3′ for chromosome 15 precursor. The putative precursor encoded on chromosome 5 was undetectable.