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. 2003 Dec;9(12):1446–1455. doi: 10.1261/rna.5152303

FIGURE 4.

FIGURE 4.

(A) Secondary structure of the UCAG tetraloop construct. Nucleotides are numbered using the numbering convention from DCloop. (B) UV melting profile of the UCAG hairpin measured at 200 mM NaCl. Data indicated as described in Figure 2C ▶. (C) Superposition of the DCloop hairpin structure with the UUCG tetraloop (PDB accession code: 1F7Y; Ennifar et al. 2000), superimposed over the UL1•GL4 base pair. The UUCG tetraloop is shown in red and the DCloop pentaloop in blue. (D) Comparison of the L1-L4 base pair and L3 nucleotide stacking interactions in the DCloop RNA (left) and UUCG tetraloop (right) structures. Nucleotides are colored as in Figure 1C ▶.