FIGURE 4.

Native gel mobility of the B. stearothermophilus trp leader transcript. 5′ ³²P-labeled RNA (1–232) was renatured under various salt conditions and fractionated on a 6% native polyacrylamide gel. Lane 1, RNA renatured in TK₀M₀; lane 2, RNA renatured in TK₁₀₀M₀; lane 3, RNA renatured in TK₁₀₀M₄. Assignments for each RNA species, based on structure mapping under these conditions (see Fig. 5 ▶), are indicated.