TABLE 1.

Summary of affinity selection yields

	H complex	A complex
Total input	125 pmole	185 pmole
No. of gradients/pooled fractions	5/20	8/32
Pooled gradient peaks	73 pmole	73 pmole
Percent gradient peak/total input	60%	40%
Input for affinity selection1	60 pmole	57 pmole
Beads after washing	34 pmole	25 pmole
Eluted proteins2	27 pmole	22 pmole
% eluted/gradient peak	37%	30%
Left on beads	7 pmole	3 pmole
Elution efficiency	80%	88%

Quantities were determined by scintillation counting and are correspondingly approximate. ¹Losses are due to adhesion of the sample to the walls of the 24-well plate and tubes. ²This includes a correction for quenching by the elution buffer.