Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fluorescein-linked adenosine nucleotide-initiated transcription. RNA products were resolved by 8% denaturing PAGE and quantitated by phosphorimaging. Transcription in the absence (lane 1) or presence (2 mM) of fluorescein derivatives (5-isomers) of adenosine nucleotides (lanes 2–13). Transcription initiation efficiency varies from 0% to 65% (indicated under the gel, depending on the linker size and hydrophobicity) under the transcription conditions.
