TABLE 2.
Comparison of selected genes affected by siRNA, poly(I):poly(C), and interferon type 1
| Gene description | siRNA | long dsRNA | IFN–α/β |
| Guanylate binding protein 1, interferon inducible (GBP1) | • | • | • |
| Cell division cycle 14 homolog A (CDC14A) | • | • | |
| Chemokine (C-C motif) ligand 2 (CCL2) | • | • | |
| Fibroblast growth factor 2 (FGF2) | • | • | |
| Proteoglycan 1, secretory granule (PRG1) | • | • | |
| Caspase 8 (CASP8) | • | • | |
| CCAAT/enhancer binding protein delta (C/EBPD) | • | • | |
| Chemokine (C-X-C motif) ligand 11 (CXCL11)a | • | • | |
| Collagen, type XVI, alpha 1 (COL16A1) | • | • | |
| Glucose monophosphate reductase (GMPR) | • | • | |
| Interferon stimulated gene 20kDa (ISG20)a | • | • | |
| Zinc finger protein 36, C3H type-like 2 (ZFP36L2) | • | • | |
| Adenosine deaminase, RNA-specific (ADAR) | • | • | |
| Complement component 1, s subcomponent (C1S) | • | • | |
| Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) | • | • | |
| Interferon regulatory factor 1 (IRF1) | • | • | |
| Interferon regulatory factor 2 (IRF2) | • | • | |
| Mothers against decapentaplegic homolog 4 (MADH4) | • | • | |
| N-myc (and STAT) interactor (NMI) | • | • | |
| Plasminogen activator, urokinase receptor (PLAUR) | • | • | |
| Transporter 1, ATP-binding cassette, subfamily B (TAP1) | • | • |
Genes found to be up- or down-regulated twofold or more by microarray analysis after treatment with a nonspecific siRNA (this study), long dsRNA [poly(I):poly(C)] (Geiss et al. 2001), or IFNα or IFNβ (Der et al. 1998).
aGenes affected by siRNA treatment that have previously been reported to be regulated by IFN (ISG20,Gongora et al. 2000; CXCL11,Cole et al. 1998) although they were not reported in the microarray analysis carried out by Der et al. (1998).