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. 2004 Jan;10(1):48–58. doi: 10.1261/rna.5154104

FIGURE 10.

FIGURE 10.

Activation of viral RNA replication using variant subgenomic RNA 4 constructs. The figure shows a Western blot for viral coat protein expression. (Lane 1) virion coat protein standard; (lane 2) mock-infected cells; (lane 3) genomic RNAs 1–3 only; (lane 4) complete inoculum containing genomic RNAs 1–3 plus subgenomic RNA 4; and (lanes 6–10) wild-type genomic RNAs 1–3 were transfected with the following variant subgenomic RNA 4 constructs: RNA 4 carrying the hairpin exchange (lane 6), RNA 4 carrying the 865A insertion (lane 7), RNA 4 carrying the 865C insertion (lane 8), RNA 4 carrying the AUGC1 insertion (lane 9), and RNA 4 carrying the 878C insertion (lane 10).