FIGURE 2.

Sequence comparison of Bacillus stearothermophilus BstDEAD, yeast eIF4A, and Methanococcus janaschii mjDEAD. BstDEAD contains all eight conserved helicase motifs in the classification of Gorbalenya and Koonin (1993). These are shown in boxes and are colored as in Figure 1 ▶. Secondary structure topology is based on DALI (Holm and Sander 1993) structural relatedness search using BstDEAD-NT model and preliminary full-length BstDEAD model, eIF4A (PDB ID no. 1FUU), and mjDEAD (PDB ID no. 1HV8). Boundaries of secondary structure elements are approximate due to variations in loop regions. Sequence alignment calculated by using CLUSTALW (Thompson et al. 1994) and generated by using ALSCRIPT (Barton 1993).