TABLE 2.

Incubation times for the initial selection for Rev ARM dependence

Round	(−) Incubation time	(+) Incubation time
1	—	16 h
2	24 h	16 h
3	24 h	5 h
4	24 h	30 min
5	48 h	5 min
6	95 h	5 min
7	95 h	1 min
8	95 h	30 sec
9	94 h	30 sec

The amount of time allowed for negative and positive selection are shown for each round. Selection conditions were as presented in the Materials and Methods section, with the exception that 25 μM substrate (instead of 10 μM) was added during the (−) selection of Round 9.