FIGURE 7.

UV cross-linking reveals that the site of interaction within RNA33–147 is at the template domain. In the sequencing gel of the cross-linking reaction between 4-thio-U hairpins and RNA33–147, each lane contains a reverse transcriptase reaction as described in Materials and Methods. Lanes 1 and 2 are the s4U7 cross-linked products run in duplicate. Lane 3 is RNA33–147 reverse transcribed with only dNTPs. Lanes U, A, G, and C correspond to the sequencing ladder produced by dideoxy sequencing methods. Lane 4 is the reverse transcriptase reaction (only dNTPs) of UV-irradiated RNA33–147. Lanes 5 and 6 are the s4U8 cross-linked products run in duplicate. Arrows indicate stops due to cross-linking. The hTR sequence of the template region is indicated alongside the gel.