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Temperature and Mg++ dependence of solvent accessibility in the class I ligase. (A) Protection factors were averaged for G45–C48 and G70–G75, and the average is plotted for experiments in which hydroxyl-radical cleavage was performed at varying temperatures in the presence of 10 mM Mg++. (B) Averaged protection factors are shown for the positions noted in panel A in experiments probing the ligase at different Mg++ concentrations (at 22°C).
