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. 2004 Feb;10(2):185–191. doi: 10.1261/rna.5167604

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FIGURE 3. — tRNA and pre-miRNA appear to bind to overlapping sites on Exp5. Binding of Exp5 from HeLa extract to immobilized tRNA and pre-miRNA was performed in the presence of RanQ69L (GTP) as described in Figure 2A ▶. Where indicated, 0.4 μM or 4 μM nonimmobilized pre-miR-31 had been added. The soluble pre-miRNA competed not only binding of Exp5 to immobilized pre-miRNA, but also to tRNA, which points to overlapping binding sites for tRNA and pre-miRNA on Exp5. The interaction between Exp-t and tRNA was not competed by pre-miRNA.