FIGURE 1.

Translation of various mRNAs by S30 extracts derived from control and cold-shocked cells after different times of cold exposure. The histograms display the amount of proteins synthesized after 15-min incubation at either 37°C (A) or 15°C (B) by equivalent amounts (normalized for their ribosome content) of E. coli MRE600 S30 extracts prepared from cells harvested before (black) and after 30-min (light gray) and 90-min (dark gray) cold shock at 10°C; the extracts were programmed with 15 pmoles each of cold shock [cspAwt, cspA(Δ1-80), and hns], cold-tolerant (P2, hupB, P3 hupB, P4hupB) and non-cold-shock (cspD and hupA) mRNAs as indicated in the abscissa. The other conditions are described in Table 1 ▶ and in Materials and Methods.