FIG. 3.

Polarity of MV transmission. Injection of MV-GFP into the CA1 region (A) results in the propagation of MV to the CA3 region and dentate gyrus (DG), whereas injection of MV-GFP into the CA3 region (B) results in transmission to the dentate gyrus but only inefficiently to the CA1 region. Fluorescence micrographs at 3, 6, and 14 dpi are shown; bottom panels (DG) show a magnification of the dentate gyrus at ∼14 dpi. The DG panel of the CA1-injected slice (A) shows a magnification that is rotated by 50° counterclockwise with regard to the upper panels and is exposed longer to better visualize GFP-positive granule cells. Bars, 20 μm (3-, 6-, and 14-day panels) and 80 μm (DG panels).