FIGURE 1.

Two-step purification scheme for the isolation of the HCV RNP complexes. The organization of the HCV subgenomic replicon is presented. Replicon RNA serves as a substrate for the hybridization of a 57-nt (5′) biotin- and (3′) digoxigenin-tagged oligonucleotide representing neomycin-resistance gene sequences in the replicon. Both sense and anti-sense neomycin oligonucleotides complementary to either plus or minus replicon RNA strands were synthesized. A flowchart illustrates the avidin-affinity and anti-digoxigenin immunoprecipitation procedures used in the purification scheme.