FIGURE 1.

Sucrose gradient patterns of modified and subsequently reassociated 50S subunits. Ribosomal particles were separated by sucrose gradient centrifugation. (A) unmodified 50S reassociated in 6 mM MgCl₂. (B) 50S, modified with CMCT were reassociated in 6 mM MgCl₂. (C) 50S, modified with 17 mM of DMS, were reassociated in 6 mM MgCl₂. (D) 50S, modified with 85 mM DMS, were reassociated in 6 mM MgCl₂. (E) 50S, modified with 85 mM DMS, were reassociated in 13 mM MgCl₂.