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Reverse transcriptase analysis of the positions of the DMS and CMCT modifications in the 23S rRNA. Positions A1912 and A1918, whose modification interfere with 50S reassociation, are denoted by arrows. The dideoxy sequencing lanes are indicated by A, C, G, and T. (+) 17 mM DMS; (++) 85 mM DMS. (Lanes 1–6) selection experiments done in 6 mM MgCl2; (lanes 7,8) selection experiments done in 13 mM MgCl2.
