FIGURE 1.

Localization of EJC proteins in adenovirus-infected cells. HeLa cells were either noninfected (A,D,G; non-inf) or infected with adenovirus for 14–16 h (B,C,E,F,H–L; Ad2). To detect nascent transcripts, cells were incubated with Br-U for 1 h (A–C). (C) A cell double-labeled for nascent transcripts (red) and viral ssDNA inclusions using anti-DBP antibody (green staining). To visualize spliceosome snRNPs, cells were immunolabeled with Y12 antibody directed against Sm proteins (D,E). Spliced viral mRNAs were detected by in situ hybridization using a probe (SJ1), which hybridizes with all mRNAs encoded by the highly expressed adenovirus major late transcription unit (Bridge et al. 1996) (F). The localization of EJC proteins was determined in cells expressing GFP–Magoh (G,H), zzY14 (I), GFP–UAP56 (J), GFP–RNPS1 (K). The distribution of SRm160 was visualized with specific rabbit polyclonal antibodies (L). Bar, 10 μm.