FIGURE 6.

EJC proteins and spliceosomal snRNPs do not colocalize with mutant β-globin nascent transcripts. MELβIVSI were induced to differentiate for 3 d, hybridized with the RNA probe (green), and labeled with antibodies against SRm160 (a), REF (b), or Sm proteins (d; red). (c) Cells were first transfected with GFP–UAP56 (green), induced to differentiate for 2d, and hybridized with the RNA probe directly labeled with Cy3 (red). The graphics in a′, b′, c′, and d′ correspond to a quantitative measurement of colocalization of the EJC proteins at the sites of mutant β-globin transcription. A line scan was made across the nucleus as indicated in the figures, and the color of the lines in the graphics matches the color of the detection of RNA and protein in the cell.