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IRF-3 5D-inducible expression of ISG56 gene. rtTA-Neo (lanes 1 to 5) and rtTA-IRF-3 5D (lanes 6 to 10) Jurkat cells were exposed to DOX (1 μg/ml) and anti-IFN antibodies for the indicated times. Whole-cell extracts (50 μg) were subjected to SDS-PAGE and analyzed by immunoblotting with anti-ISG56 antibodies. Membranes were stripped and reprobed with anti-IRF-3 and anti-actin antibodies.
