FIGURE 1.
Human mitochondrial wild-type and pseudo-WT tRNALys transcripts. (A) Determined secondary structures of human mt wild-type tRNALys transcript (Kwt; Helm et al. 1998). (B) Experimental secondary cloverleaf structures of native mt tRNALys (displaying the six post-transcriptional modifications, abbreviated in the conventional way; Sprinzl et al. 1998) and of a chimeric tRNALys methylated at position N1 of A9 as sole post-transcriptional modification (Helm et al. 1999b). (C) Pseudo-WT in vitro transcribed tRNALys derivatives Ke and Ki. tRNA domains are labeled for Ke. Structural mutations introduced at positions 50 and 64 (indicated by gray circles) prevent alternate folding into extended hairpin (black crosses; Helm et al. 1998).